volocity 3d image analysis version 6.5.1 (Quorum Technologies)
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volocity 3d image analysis version 6.5.1
Volocity 3d Image Analysis Version 6.5.1, supplied by Quorum Technologies, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/volocity+3d+image+analysis+version+6%2E5%2E1/volocity+software/pmc10945611-57-3-10
Average 90 stars, based on 1 article reviews
Volocity 3d Image Analysis Version 6.5.1, supplied by Quorum Technologies, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/volocity+3d+image+analysis+version+6%2E5%2E1/volocity+software/pmc10945611-57-3-10
Average 90 stars, based on 1 article reviews
volocity 3d image analysis version 6.5.1 - by Bioz Stars,
2026-10
90/100 stars
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1) Product Images from "Downregulation of Dickkopf-3, a Wnt antagonist elevated in Alzheimer’s disease, restores synapse integrity and memory in a disease mouse model"
Article Title: Downregulation of Dickkopf-3, a Wnt antagonist elevated in Alzheimer’s disease, restores synapse integrity and memory in a disease mouse model
Journal: eLife
doi: 10.7554/eLife.89453
Figure Legend Snippet:
Techniques Used: Isolation, Knock-Out, Control, Sequencing, shRNA, Recombinant, TUNEL Assay, cDNA Synthesis, Fluorsave, Software, Microscopy, Immunofluorescence, Patch Clamp
Related Articles
Software:Article Title: Downregulation of Dickkopf-3, a Wnt antagonist elevated in Alzheimer’s disease, restores synapse integrity and memory in a disease mouse model Article Snippet: Software, algorithm , Article Title: Downregulation of Dickkopf-3, a Wnt antagonist elevated in Alzheimer’s disease, restores synapse integrity and memory in a disease mouse model Article Snippet: Primary antibodies and dilutions used for immunofluorescence were: mouse Anti- Aβ (6E10 clone, 1:1000, Biolegend, Cat# 803001, RRID: AB_2564653 ), Rabbit Anti-Aβ (6E10 clone, chimeric, 1:1000, Novus Biologicals, Cat# NBP2-62566, RRID: AB_2917960 ), chicken anti-Bassoon (1:1,000, Synaptic Systems, Cat# 141 016, RRID: AB_2661779 ), Goat anti- Mouse DKK-3 (1:1000, R and D Systems, Cat# AF948, RRID: AB_355734 ), Rabbit anti-Gephyrin (1:500, Synaptic Systems, Cat# 147 002, RRID: AB_2619838 ), Chicken anti-Glial Fibrillary Acidic Protein (GFAP) (1:500, Millipore, Cat# AB5541, RRID: AB_177521 ), Rabbit anti-Homer1 (1:1000, Synaptic Systems, Cat# 160 003, RRID: AB_887730 ), chicken Anti-GFP (1:500, Millipore, Cat# 06–896, RRID: AB_310288 ), rabbit anti-Iba1 (1:1000, FUJIFILM Wako Shibayagi, Cat# 019–1974,1 RRID: AB_839504 ), rat anti-LAMP1 (1:250, DSHB, 1D4B, RRID: AB_528127 ), rabbit Anti-NeuN (D3S3I) (1:1000, Cell Signaling Technology, Cat# 12943, RRID: AB_2630395 ), rabbit Anti-Neurofilament Heavy (1:5000, Abcam, Cat# ab8135, RRID: AB_306298 ), mouse Anti-PSD-95 (7E3-1B8) (1:500, Thermo Fisher Scientific, Cat# MA1-046, RRID: AB_2092361 ), guinea pig anti-vesicular GABA Transporter (vGAT) (1:500, Synaptic Systems, Cat# 131 004, RRID: AB_887873 ), guinea pig Anti-Vesicular Glutamate Transporter 1 (vGLUT1) (1:2000, Millipore, Cat# AB5905, RRID: AB_2301751 ), goat anti-Wnt7a/b (1:1000, R and D Systems, Cat# AF3460, RRID: AB_2304437 ), mouse Anti- beta Catenin (1:1000, BD Biosciences, Cat# 610153, RRID: AB_397554 ). Isolation:Article Title: Downregulation of Dickkopf-3, a Wnt antagonist elevated in Alzheimer’s disease, restores synapse integrity and memory in a disease mouse model Article Snippet: Software, algorithm , Article Title: Downregulation of Dickkopf-3, a Wnt antagonist elevated in Alzheimer’s disease, restores synapse integrity and memory in a disease mouse model Article Snippet: Primary antibodies and dilutions used for immunofluorescence were: mouse Anti- Aβ (6E10 clone, 1:1000, Biolegend, Cat# 803001, RRID: AB_2564653 ), Rabbit Anti-Aβ (6E10 clone, chimeric, 1:1000, Novus Biologicals, Cat# NBP2-62566, RRID: AB_2917960 ), chicken anti-Bassoon (1:1,000, Synaptic Systems, Cat# 141 016, RRID: AB_2661779 ), Goat anti- Mouse DKK-3 (1:1000, R and D Systems, Cat# AF948, RRID: AB_355734 ), Rabbit anti-Gephyrin (1:500, Synaptic Systems, Cat# 147 002, RRID: AB_2619838 ), Chicken anti-Glial Fibrillary Acidic Protein (GFAP) (1:500, Millipore, Cat# AB5541, RRID: AB_177521 ), Rabbit anti-Homer1 (1:1000, Synaptic Systems, Cat# 160 003, RRID: AB_887730 ), chicken Anti-GFP (1:500, Millipore, Cat# 06–896, RRID: AB_310288 ), rabbit anti-Iba1 (1:1000, FUJIFILM Wako Shibayagi, Cat# 019–1974,1 RRID: AB_839504 ), rat anti-LAMP1 (1:250, DSHB, 1D4B, RRID: AB_528127 ), rabbit Anti-NeuN (D3S3I) (1:1000, Cell Signaling Technology, Cat# 12943, RRID: AB_2630395 ), rabbit Anti-Neurofilament Heavy (1:5000, Abcam, Cat# ab8135, RRID: AB_306298 ), mouse Anti-PSD-95 (7E3-1B8) (1:500, Thermo Fisher Scientific, Cat# MA1-046, RRID: AB_2092361 ), guinea pig anti-vesicular GABA Transporter (vGAT) (1:500, Synaptic Systems, Cat# 131 004, RRID: AB_887873 ), guinea pig Anti-Vesicular Glutamate Transporter 1 (vGLUT1) (1:2000, Millipore, Cat# AB5905, RRID: AB_2301751 ), goat anti-Wnt7a/b (1:1000, R and D Systems, Cat# AF3460, RRID: AB_2304437 ), mouse Anti- beta Catenin (1:1000, BD Biosciences, Cat# 610153, RRID: AB_397554 ). Knock-Out:Article Title: Downregulation of Dickkopf-3, a Wnt antagonist elevated in Alzheimer’s disease, restores synapse integrity and memory in a disease mouse model Article Snippet: Software, algorithm , Article Title: Downregulation of Dickkopf-3, a Wnt antagonist elevated in Alzheimer’s disease, restores synapse integrity and memory in a disease mouse model Article Snippet: Primary antibodies and dilutions used for immunofluorescence were: mouse Anti- Aβ (6E10 clone, 1:1000, Biolegend, Cat# 803001, RRID: AB_2564653 ), Rabbit Anti-Aβ (6E10 clone, chimeric, 1:1000, Novus Biologicals, Cat# NBP2-62566, RRID: AB_2917960 ), chicken anti-Bassoon (1:1,000, Synaptic Systems, Cat# 141 016, RRID: AB_2661779 ), Goat anti- Mouse DKK-3 (1:1000, R and D Systems, Cat# AF948, RRID: AB_355734 ), Rabbit anti-Gephyrin (1:500, Synaptic Systems, Cat# 147 002, RRID: AB_2619838 ), Chicken anti-Glial Fibrillary Acidic Protein (GFAP) (1:500, Millipore, Cat# AB5541, RRID: AB_177521 ), Rabbit anti-Homer1 (1:1000, Synaptic Systems, Cat# 160 003, RRID: AB_887730 ), chicken Anti-GFP (1:500, Millipore, Cat# 06–896, RRID: AB_310288 ), rabbit anti-Iba1 (1:1000, FUJIFILM Wako Shibayagi, Cat# 019–1974,1 RRID: AB_839504 ), rat anti-LAMP1 (1:250, DSHB, 1D4B, RRID: AB_528127 ), rabbit Anti-NeuN (D3S3I) (1:1000, Cell Signaling Technology, Cat# 12943, RRID: AB_2630395 ), rabbit Anti-Neurofilament Heavy (1:5000, Abcam, Cat# ab8135, RRID: AB_306298 ), mouse Anti-PSD-95 (7E3-1B8) (1:500, Thermo Fisher Scientific, Cat# MA1-046, RRID: AB_2092361 ), guinea pig anti-vesicular GABA Transporter (vGAT) (1:500, Synaptic Systems, Cat# 131 004, RRID: AB_887873 ), guinea pig Anti-Vesicular Glutamate Transporter 1 (vGLUT1) (1:2000, Millipore, Cat# AB5905, RRID: AB_2301751 ), goat anti-Wnt7a/b (1:1000, R and D Systems, Cat# AF3460, RRID: AB_2304437 ), mouse Anti- beta Catenin (1:1000, BD Biosciences, Cat# 610153, RRID: AB_397554 ). Control:Article Title: Downregulation of Dickkopf-3, a Wnt antagonist elevated in Alzheimer’s disease, restores synapse integrity and memory in a disease mouse model Article Snippet: Software, algorithm , Article Title: Downregulation of Dickkopf-3, a Wnt antagonist elevated in Alzheimer’s disease, restores synapse integrity and memory in a disease mouse model Article Snippet: Primary antibodies and dilutions used for immunofluorescence were: mouse Anti- Aβ (6E10 clone, 1:1000, Biolegend, Cat# 803001, RRID: AB_2564653 ), Rabbit Anti-Aβ (6E10 clone, chimeric, 1:1000, Novus Biologicals, Cat# NBP2-62566, RRID: AB_2917960 ), chicken anti-Bassoon (1:1,000, Synaptic Systems, Cat# 141 016, RRID: AB_2661779 ), Goat anti- Mouse DKK-3 (1:1000, R and D Systems, Cat# AF948, RRID: AB_355734 ), Rabbit anti-Gephyrin (1:500, Synaptic Systems, Cat# 147 002, RRID: AB_2619838 ), Chicken anti-Glial Fibrillary Acidic Protein (GFAP) (1:500, Millipore, Cat# AB5541, RRID: AB_177521 ), Rabbit anti-Homer1 (1:1000, Synaptic Systems, Cat# 160 003, RRID: AB_887730 ), chicken Anti-GFP (1:500, Millipore, Cat# 06–896, RRID: AB_310288 ), rabbit anti-Iba1 (1:1000, FUJIFILM Wako Shibayagi, Cat# 019–1974,1 RRID: AB_839504 ), rat anti-LAMP1 (1:250, DSHB, 1D4B, RRID: AB_528127 ), rabbit Anti-NeuN (D3S3I) (1:1000, Cell Signaling Technology, Cat# 12943, RRID: AB_2630395 ), rabbit Anti-Neurofilament Heavy (1:5000, Abcam, Cat# ab8135, RRID: AB_306298 ), mouse Anti-PSD-95 (7E3-1B8) (1:500, Thermo Fisher Scientific, Cat# MA1-046, RRID: AB_2092361 ), guinea pig anti-vesicular GABA Transporter (vGAT) (1:500, Synaptic Systems, Cat# 131 004, RRID: AB_887873 ), guinea pig Anti-Vesicular Glutamate Transporter 1 (vGLUT1) (1:2000, Millipore, Cat# AB5905, RRID: AB_2301751 ), goat anti-Wnt7a/b (1:1000, R and D Systems, Cat# AF3460, RRID: AB_2304437 ), mouse Anti- beta Catenin (1:1000, BD Biosciences, Cat# 610153, RRID: AB_397554 ). Sequencing:Article Title: Downregulation of Dickkopf-3, a Wnt antagonist elevated in Alzheimer’s disease, restores synapse integrity and memory in a disease mouse model Article Snippet: Software, algorithm , Article Title: Downregulation of Dickkopf-3, a Wnt antagonist elevated in Alzheimer’s disease, restores synapse integrity and memory in a disease mouse model Article Snippet: Primary antibodies and dilutions used for immunofluorescence were: mouse Anti- Aβ (6E10 clone, 1:1000, Biolegend, Cat# 803001, RRID: AB_2564653 ), Rabbit Anti-Aβ (6E10 clone, chimeric, 1:1000, Novus Biologicals, Cat# NBP2-62566, RRID: AB_2917960 ), chicken anti-Bassoon (1:1,000, Synaptic Systems, Cat# 141 016, RRID: AB_2661779 ), Goat anti- Mouse DKK-3 (1:1000, R and D Systems, Cat# AF948, RRID: AB_355734 ), Rabbit anti-Gephyrin (1:500, Synaptic Systems, Cat# 147 002, RRID: AB_2619838 ), Chicken anti-Glial Fibrillary Acidic Protein (GFAP) (1:500, Millipore, Cat# AB5541, RRID: AB_177521 ), Rabbit anti-Homer1 (1:1000, Synaptic Systems, Cat# 160 003, RRID: AB_887730 ), chicken Anti-GFP (1:500, Millipore, Cat# 06–896, RRID: AB_310288 ), rabbit anti-Iba1 (1:1000, FUJIFILM Wako Shibayagi, Cat# 019–1974,1 RRID: AB_839504 ), rat anti-LAMP1 (1:250, DSHB, 1D4B, RRID: AB_528127 ), rabbit Anti-NeuN (D3S3I) (1:1000, Cell Signaling Technology, Cat# 12943, RRID: AB_2630395 ), rabbit Anti-Neurofilament Heavy (1:5000, Abcam, Cat# ab8135, RRID: AB_306298 ), mouse Anti-PSD-95 (7E3-1B8) (1:500, Thermo Fisher Scientific, Cat# MA1-046, RRID: AB_2092361 ), guinea pig anti-vesicular GABA Transporter (vGAT) (1:500, Synaptic Systems, Cat# 131 004, RRID: AB_887873 ), guinea pig Anti-Vesicular Glutamate Transporter 1 (vGLUT1) (1:2000, Millipore, Cat# AB5905, RRID: AB_2301751 ), goat anti-Wnt7a/b (1:1000, R and D Systems, Cat# AF3460, RRID: AB_2304437 ), mouse Anti- beta Catenin (1:1000, BD Biosciences, Cat# 610153, RRID: AB_397554 ). shRNA:Article Title: Downregulation of Dickkopf-3, a Wnt antagonist elevated in Alzheimer’s disease, restores synapse integrity and memory in a disease mouse model Article Snippet: Software, algorithm , Article Title: Downregulation of Dickkopf-3, a Wnt antagonist elevated in Alzheimer’s disease, restores synapse integrity and memory in a disease mouse model Article Snippet: Primary antibodies and dilutions used for immunofluorescence were: mouse Anti- Aβ (6E10 clone, 1:1000, Biolegend, Cat# 803001, RRID: AB_2564653 ), Rabbit Anti-Aβ (6E10 clone, chimeric, 1:1000, Novus Biologicals, Cat# NBP2-62566, RRID: AB_2917960 ), chicken anti-Bassoon (1:1,000, Synaptic Systems, Cat# 141 016, RRID: AB_2661779 ), Goat anti- Mouse DKK-3 (1:1000, R and D Systems, Cat# AF948, RRID: AB_355734 ), Rabbit anti-Gephyrin (1:500, Synaptic Systems, Cat# 147 002, RRID: AB_2619838 ), Chicken anti-Glial Fibrillary Acidic Protein (GFAP) (1:500, Millipore, Cat# AB5541, RRID: AB_177521 ), Rabbit anti-Homer1 (1:1000, Synaptic Systems, Cat# 160 003, RRID: AB_887730 ), chicken Anti-GFP (1:500, Millipore, Cat# 06–896, RRID: AB_310288 ), rabbit anti-Iba1 (1:1000, FUJIFILM Wako Shibayagi, Cat# 019–1974,1 RRID: AB_839504 ), rat anti-LAMP1 (1:250, DSHB, 1D4B, RRID: AB_528127 ), rabbit Anti-NeuN (D3S3I) (1:1000, Cell Signaling Technology, Cat# 12943, RRID: AB_2630395 ), rabbit Anti-Neurofilament Heavy (1:5000, Abcam, Cat# ab8135, RRID: AB_306298 ), mouse Anti-PSD-95 (7E3-1B8) (1:500, Thermo Fisher Scientific, Cat# MA1-046, RRID: AB_2092361 ), guinea pig anti-vesicular GABA Transporter (vGAT) (1:500, Synaptic Systems, Cat# 131 004, RRID: AB_887873 ), guinea pig Anti-Vesicular Glutamate Transporter 1 (vGLUT1) (1:2000, Millipore, Cat# AB5905, RRID: AB_2301751 ), goat anti-Wnt7a/b (1:1000, R and D Systems, Cat# AF3460, RRID: AB_2304437 ), mouse Anti- beta Catenin (1:1000, BD Biosciences, Cat# 610153, RRID: AB_397554 ). Recombinant:Article Title: Downregulation of Dickkopf-3, a Wnt antagonist elevated in Alzheimer’s disease, restores synapse integrity and memory in a disease mouse model Article Snippet: Software, algorithm , Article Title: Downregulation of Dickkopf-3, a Wnt antagonist elevated in Alzheimer’s disease, restores synapse integrity and memory in a disease mouse model Article Snippet: Primary antibodies and dilutions used for immunofluorescence were: mouse Anti- Aβ (6E10 clone, 1:1000, Biolegend, Cat# 803001, RRID: AB_2564653 ), Rabbit Anti-Aβ (6E10 clone, chimeric, 1:1000, Novus Biologicals, Cat# NBP2-62566, RRID: AB_2917960 ), chicken anti-Bassoon (1:1,000, Synaptic Systems, Cat# 141 016, RRID: AB_2661779 ), Goat anti- Mouse DKK-3 (1:1000, R and D Systems, Cat# AF948, RRID: AB_355734 ), Rabbit anti-Gephyrin (1:500, Synaptic Systems, Cat# 147 002, RRID: AB_2619838 ), Chicken anti-Glial Fibrillary Acidic Protein (GFAP) (1:500, Millipore, Cat# AB5541, RRID: AB_177521 ), Rabbit anti-Homer1 (1:1000, Synaptic Systems, Cat# 160 003, RRID: AB_887730 ), chicken Anti-GFP (1:500, Millipore, Cat# 06–896, RRID: AB_310288 ), rabbit anti-Iba1 (1:1000, FUJIFILM Wako Shibayagi, Cat# 019–1974,1 RRID: AB_839504 ), rat anti-LAMP1 (1:250, DSHB, 1D4B, RRID: AB_528127 ), rabbit Anti-NeuN (D3S3I) (1:1000, Cell Signaling Technology, Cat# 12943, RRID: AB_2630395 ), rabbit Anti-Neurofilament Heavy (1:5000, Abcam, Cat# ab8135, RRID: AB_306298 ), mouse Anti-PSD-95 (7E3-1B8) (1:500, Thermo Fisher Scientific, Cat# MA1-046, RRID: AB_2092361 ), guinea pig anti-vesicular GABA Transporter (vGAT) (1:500, Synaptic Systems, Cat# 131 004, RRID: AB_887873 ), guinea pig Anti-Vesicular Glutamate Transporter 1 (vGLUT1) (1:2000, Millipore, Cat# AB5905, RRID: AB_2301751 ), goat anti-Wnt7a/b (1:1000, R and D Systems, Cat# AF3460, RRID: AB_2304437 ), mouse Anti- beta Catenin (1:1000, BD Biosciences, Cat# 610153, RRID: AB_397554 ). TUNEL Assay:Article Title: Downregulation of Dickkopf-3, a Wnt antagonist elevated in Alzheimer’s disease, restores synapse integrity and memory in a disease mouse model Article Snippet: Software, algorithm , Article Title: Downregulation of Dickkopf-3, a Wnt antagonist elevated in Alzheimer’s disease, restores synapse integrity and memory in a disease mouse model Article Snippet: Primary antibodies and dilutions used for immunofluorescence were: mouse Anti- Aβ (6E10 clone, 1:1000, Biolegend, Cat# 803001, RRID: AB_2564653 ), Rabbit Anti-Aβ (6E10 clone, chimeric, 1:1000, Novus Biologicals, Cat# NBP2-62566, RRID: AB_2917960 ), chicken anti-Bassoon (1:1,000, Synaptic Systems, Cat# 141 016, RRID: AB_2661779 ), Goat anti- Mouse DKK-3 (1:1000, R and D Systems, Cat# AF948, RRID: AB_355734 ), Rabbit anti-Gephyrin (1:500, Synaptic Systems, Cat# 147 002, RRID: AB_2619838 ), Chicken anti-Glial Fibrillary Acidic Protein (GFAP) (1:500, Millipore, Cat# AB5541, RRID: AB_177521 ), Rabbit anti-Homer1 (1:1000, Synaptic Systems, Cat# 160 003, RRID: AB_887730 ), chicken Anti-GFP (1:500, Millipore, Cat# 06–896, RRID: AB_310288 ), rabbit anti-Iba1 (1:1000, FUJIFILM Wako Shibayagi, Cat# 019–1974,1 RRID: AB_839504 ), rat anti-LAMP1 (1:250, DSHB, 1D4B, RRID: AB_528127 ), rabbit Anti-NeuN (D3S3I) (1:1000, Cell Signaling Technology, Cat# 12943, RRID: AB_2630395 ), rabbit Anti-Neurofilament Heavy (1:5000, Abcam, Cat# ab8135, RRID: AB_306298 ), mouse Anti-PSD-95 (7E3-1B8) (1:500, Thermo Fisher Scientific, Cat# MA1-046, RRID: AB_2092361 ), guinea pig anti-vesicular GABA Transporter (vGAT) (1:500, Synaptic Systems, Cat# 131 004, RRID: AB_887873 ), guinea pig Anti-Vesicular Glutamate Transporter 1 (vGLUT1) (1:2000, Millipore, Cat# AB5905, RRID: AB_2301751 ), goat anti-Wnt7a/b (1:1000, R and D Systems, Cat# AF3460, RRID: AB_2304437 ), mouse Anti- beta Catenin (1:1000, BD Biosciences, Cat# 610153, RRID: AB_397554 ). cDNA Synthesis:Article Title: Downregulation of Dickkopf-3, a Wnt antagonist elevated in Alzheimer’s disease, restores synapse integrity and memory in a disease mouse model Article Snippet: Software, algorithm , Article Title: Downregulation of Dickkopf-3, a Wnt antagonist elevated in Alzheimer’s disease, restores synapse integrity and memory in a disease mouse model Article Snippet: Primary antibodies and dilutions used for immunofluorescence were: mouse Anti- Aβ (6E10 clone, 1:1000, Biolegend, Cat# 803001, RRID: AB_2564653 ), Rabbit Anti-Aβ (6E10 clone, chimeric, 1:1000, Novus Biologicals, Cat# NBP2-62566, RRID: AB_2917960 ), chicken anti-Bassoon (1:1,000, Synaptic Systems, Cat# 141 016, RRID: AB_2661779 ), Goat anti- Mouse DKK-3 (1:1000, R and D Systems, Cat# AF948, RRID: AB_355734 ), Rabbit anti-Gephyrin (1:500, Synaptic Systems, Cat# 147 002, RRID: AB_2619838 ), Chicken anti-Glial Fibrillary Acidic Protein (GFAP) (1:500, Millipore, Cat# AB5541, RRID: AB_177521 ), Rabbit anti-Homer1 (1:1000, Synaptic Systems, Cat# 160 003, RRID: AB_887730 ), chicken Anti-GFP (1:500, Millipore, Cat# 06–896, RRID: AB_310288 ), rabbit anti-Iba1 (1:1000, FUJIFILM Wako Shibayagi, Cat# 019–1974,1 RRID: AB_839504 ), rat anti-LAMP1 (1:250, DSHB, 1D4B, RRID: AB_528127 ), rabbit Anti-NeuN (D3S3I) (1:1000, Cell Signaling Technology, Cat# 12943, RRID: AB_2630395 ), rabbit Anti-Neurofilament Heavy (1:5000, Abcam, Cat# ab8135, RRID: AB_306298 ), mouse Anti-PSD-95 (7E3-1B8) (1:500, Thermo Fisher Scientific, Cat# MA1-046, RRID: AB_2092361 ), guinea pig anti-vesicular GABA Transporter (vGAT) (1:500, Synaptic Systems, Cat# 131 004, RRID: AB_887873 ), guinea pig Anti-Vesicular Glutamate Transporter 1 (vGLUT1) (1:2000, Millipore, Cat# AB5905, RRID: AB_2301751 ), goat anti-Wnt7a/b (1:1000, R and D Systems, Cat# AF3460, RRID: AB_2304437 ), mouse Anti- beta Catenin (1:1000, BD Biosciences, Cat# 610153, RRID: AB_397554 ). Fluorsave:Article Title: Downregulation of Dickkopf-3, a Wnt antagonist elevated in Alzheimer’s disease, restores synapse integrity and memory in a disease mouse model Article Snippet: Software, algorithm , Article Title: Downregulation of Dickkopf-3, a Wnt antagonist elevated in Alzheimer’s disease, restores synapse integrity and memory in a disease mouse model Article Snippet: Primary antibodies and dilutions used for immunofluorescence were: mouse Anti- Aβ (6E10 clone, 1:1000, Biolegend, Cat# 803001, RRID: AB_2564653 ), Rabbit Anti-Aβ (6E10 clone, chimeric, 1:1000, Novus Biologicals, Cat# NBP2-62566, RRID: AB_2917960 ), chicken anti-Bassoon (1:1,000, Synaptic Systems, Cat# 141 016, RRID: AB_2661779 ), Goat anti- Mouse DKK-3 (1:1000, R and D Systems, Cat# AF948, RRID: AB_355734 ), Rabbit anti-Gephyrin (1:500, Synaptic Systems, Cat# 147 002, RRID: AB_2619838 ), Chicken anti-Glial Fibrillary Acidic Protein (GFAP) (1:500, Millipore, Cat# AB5541, RRID: AB_177521 ), Rabbit anti-Homer1 (1:1000, Synaptic Systems, Cat# 160 003, RRID: AB_887730 ), chicken Anti-GFP (1:500, Millipore, Cat# 06–896, RRID: AB_310288 ), rabbit anti-Iba1 (1:1000, FUJIFILM Wako Shibayagi, Cat# 019–1974,1 RRID: AB_839504 ), rat anti-LAMP1 (1:250, DSHB, 1D4B, RRID: AB_528127 ), rabbit Anti-NeuN (D3S3I) (1:1000, Cell Signaling Technology, Cat# 12943, RRID: AB_2630395 ), rabbit Anti-Neurofilament Heavy (1:5000, Abcam, Cat# ab8135, RRID: AB_306298 ), mouse Anti-PSD-95 (7E3-1B8) (1:500, Thermo Fisher Scientific, Cat# MA1-046, RRID: AB_2092361 ), guinea pig anti-vesicular GABA Transporter (vGAT) (1:500, Synaptic Systems, Cat# 131 004, RRID: AB_887873 ), guinea pig Anti-Vesicular Glutamate Transporter 1 (vGLUT1) (1:2000, Millipore, Cat# AB5905, RRID: AB_2301751 ), goat anti-Wnt7a/b (1:1000, R and D Systems, Cat# AF3460, RRID: AB_2304437 ), mouse Anti- beta Catenin (1:1000, BD Biosciences, Cat# 610153, RRID: AB_397554 ). Microscopy:Article Title: Downregulation of Dickkopf-3, a Wnt antagonist elevated in Alzheimer’s disease, restores synapse integrity and memory in a disease mouse model Article Snippet: Software, algorithm , Article Title: Downregulation of Dickkopf-3, a Wnt antagonist elevated in Alzheimer’s disease, restores synapse integrity and memory in a disease mouse model Article Snippet: Primary antibodies and dilutions used for immunofluorescence were: mouse Anti- Aβ (6E10 clone, 1:1000, Biolegend, Cat# 803001, RRID: AB_2564653 ), Rabbit Anti-Aβ (6E10 clone, chimeric, 1:1000, Novus Biologicals, Cat# NBP2-62566, RRID: AB_2917960 ), chicken anti-Bassoon (1:1,000, Synaptic Systems, Cat# 141 016, RRID: AB_2661779 ), Goat anti- Mouse DKK-3 (1:1000, R and D Systems, Cat# AF948, RRID: AB_355734 ), Rabbit anti-Gephyrin (1:500, Synaptic Systems, Cat# 147 002, RRID: AB_2619838 ), Chicken anti-Glial Fibrillary Acidic Protein (GFAP) (1:500, Millipore, Cat# AB5541, RRID: AB_177521 ), Rabbit anti-Homer1 (1:1000, Synaptic Systems, Cat# 160 003, RRID: AB_887730 ), chicken Anti-GFP (1:500, Millipore, Cat# 06–896, RRID: AB_310288 ), rabbit anti-Iba1 (1:1000, FUJIFILM Wako Shibayagi, Cat# 019–1974,1 RRID: AB_839504 ), rat anti-LAMP1 (1:250, DSHB, 1D4B, RRID: AB_528127 ), rabbit Anti-NeuN (D3S3I) (1:1000, Cell Signaling Technology, Cat# 12943, RRID: AB_2630395 ), rabbit Anti-Neurofilament Heavy (1:5000, Abcam, Cat# ab8135, RRID: AB_306298 ), mouse Anti-PSD-95 (7E3-1B8) (1:500, Thermo Fisher Scientific, Cat# MA1-046, RRID: AB_2092361 ), guinea pig anti-vesicular GABA Transporter (vGAT) (1:500, Synaptic Systems, Cat# 131 004, RRID: AB_887873 ), guinea pig Anti-Vesicular Glutamate Transporter 1 (vGLUT1) (1:2000, Millipore, Cat# AB5905, RRID: AB_2301751 ), goat anti-Wnt7a/b (1:1000, R and D Systems, Cat# AF3460, RRID: AB_2304437 ), mouse Anti- beta Catenin (1:1000, BD Biosciences, Cat# 610153, RRID: AB_397554 ). Immunofluorescence:Article Title: Downregulation of Dickkopf-3, a Wnt antagonist elevated in Alzheimer’s disease, restores synapse integrity and memory in a disease mouse model Article Snippet: Software, algorithm , Article Title: Downregulation of Dickkopf-3, a Wnt antagonist elevated in Alzheimer’s disease, restores synapse integrity and memory in a disease mouse model Article Snippet: Primary antibodies and dilutions used for immunofluorescence were: mouse Anti- Aβ (6E10 clone, 1:1000, Biolegend, Cat# 803001, RRID: AB_2564653 ), Rabbit Anti-Aβ (6E10 clone, chimeric, 1:1000, Novus Biologicals, Cat# NBP2-62566, RRID: AB_2917960 ), chicken anti-Bassoon (1:1,000, Synaptic Systems, Cat# 141 016, RRID: AB_2661779 ), Goat anti- Mouse DKK-3 (1:1000, R and D Systems, Cat# AF948, RRID: AB_355734 ), Rabbit anti-Gephyrin (1:500, Synaptic Systems, Cat# 147 002, RRID: AB_2619838 ), Chicken anti-Glial Fibrillary Acidic Protein (GFAP) (1:500, Millipore, Cat# AB5541, RRID: AB_177521 ), Rabbit anti-Homer1 (1:1000, Synaptic Systems, Cat# 160 003, RRID: AB_887730 ), chicken Anti-GFP (1:500, Millipore, Cat# 06–896, RRID: AB_310288 ), rabbit anti-Iba1 (1:1000, FUJIFILM Wako Shibayagi, Cat# 019–1974,1 RRID: AB_839504 ), rat anti-LAMP1 (1:250, DSHB, 1D4B, RRID: AB_528127 ), rabbit Anti-NeuN (D3S3I) (1:1000, Cell Signaling Technology, Cat# 12943, RRID: AB_2630395 ), rabbit Anti-Neurofilament Heavy (1:5000, Abcam, Cat# ab8135, RRID: AB_306298 ), mouse Anti-PSD-95 (7E3-1B8) (1:500, Thermo Fisher Scientific, Cat# MA1-046, RRID: AB_2092361 ), guinea pig anti-vesicular GABA Transporter (vGAT) (1:500, Synaptic Systems, Cat# 131 004, RRID: AB_887873 ), guinea pig Anti-Vesicular Glutamate Transporter 1 (vGLUT1) (1:2000, Millipore, Cat# AB5905, RRID: AB_2301751 ), goat anti-Wnt7a/b (1:1000, R and D Systems, Cat# AF3460, RRID: AB_2304437 ), mouse Anti- beta Catenin (1:1000, BD Biosciences, Cat# 610153, RRID: AB_397554 ). Patch Clamp:Article Title: Downregulation of Dickkopf-3, a Wnt antagonist elevated in Alzheimer’s disease, restores synapse integrity and memory in a disease mouse model Article Snippet: Software, algorithm , Article Title: Downregulation of Dickkopf-3, a Wnt antagonist elevated in Alzheimer’s disease, restores synapse integrity and memory in a disease mouse model Article Snippet: Primary antibodies and dilutions used for immunofluorescence were: mouse Anti- Aβ (6E10 clone, 1:1000, Biolegend, Cat# 803001, RRID: AB_2564653 ), Rabbit Anti-Aβ (6E10 clone, chimeric, 1:1000, Novus Biologicals, Cat# NBP2-62566, RRID: AB_2917960 ), chicken anti-Bassoon (1:1,000, Synaptic Systems, Cat# 141 016, RRID: AB_2661779 ), Goat anti- Mouse DKK-3 (1:1000, R and D Systems, Cat# AF948, RRID: AB_355734 ), Rabbit anti-Gephyrin (1:500, Synaptic Systems, Cat# 147 002, RRID: AB_2619838 ), Chicken anti-Glial Fibrillary Acidic Protein (GFAP) (1:500, Millipore, Cat# AB5541, RRID: AB_177521 ), Rabbit anti-Homer1 (1:1000, Synaptic Systems, Cat# 160 003, RRID: AB_887730 ), chicken Anti-GFP (1:500, Millipore, Cat# 06–896, RRID: AB_310288 ), rabbit anti-Iba1 (1:1000, FUJIFILM Wako Shibayagi, Cat# 019–1974,1 RRID: AB_839504 ), rat anti-LAMP1 (1:250, DSHB, 1D4B, RRID: AB_528127 ), rabbit Anti-NeuN (D3S3I) (1:1000, Cell Signaling Technology, Cat# 12943, RRID: AB_2630395 ), rabbit Anti-Neurofilament Heavy (1:5000, Abcam, Cat# ab8135, RRID: AB_306298 ), mouse Anti-PSD-95 (7E3-1B8) (1:500, Thermo Fisher Scientific, Cat# MA1-046, RRID: AB_2092361 ), guinea pig anti-vesicular GABA Transporter (vGAT) (1:500, Synaptic Systems, Cat# 131 004, RRID: AB_887873 ), guinea pig Anti-Vesicular Glutamate Transporter 1 (vGLUT1) (1:2000, Millipore, Cat# AB5905, RRID: AB_2301751 ), goat anti-Wnt7a/b (1:1000, R and D Systems, Cat# AF3460, RRID: AB_2304437 ), mouse Anti- beta Catenin (1:1000, BD Biosciences, Cat# 610153, RRID: AB_397554 ). |